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发明授权
CN105907826B 一种植物多肽/蛋白的清洁制备方法 有权

一种植物多肽/蛋白的清洁制备方法
摘要：
本发明公开了一种分子量区间可控的植物多肽/蛋白的清洁制备方法，包括以下步骤：(1)原料预处理；(2)调浆；(3)提取与分离；(4)净化，得蛋白液；(5)酶解：将上述蛋白液加热变性；加入Na2SO3，再加入蛋白酶；蛋白酶选自：Alcalase碱性蛋白酶，AS1398中性蛋白酶，胃蛋白酶，木瓜蛋白酶；(6)多肽脱苦；(7)用纳滤膜进行分离，得多肽溶液；(8)干燥失水得多肽产品。通过该方法制备得到的产品，分子量区间可控，具有高溶解性、低粘度、酸溶稳定性、低苦味等特性。本发明的产品可被广泛应用于食品、运动食品、疗效保健食品、蛋白饮料、减肥食品、新型发酵食品中。
摘要（英）：

The invention discloses a clean preparation method for plant polypeptide/protein with a controllable molecular weight interval. The method comprises the following steps of 1, pretreatment of raw materials; 2, pulp mixing; 3, extraction and separation; 4, purification to obtain protein liquid; 5, enzymolysis, wherein the protein liquid is heated for denaturation, Na2SO3 is added, then protease is added, and protease is selected from Alcalase, neutral protease AS1398, pepsin and papain; 6, debittering of polypeptide; 7, separation through a nanofiltration membrane to obtain a polypeptide solution; 8, drying and dewatering to obtain the polypeptide product. The product prepared through the method has the controllable molecular weight interval and the advantages of high solubility, low viscosity, acid soluble stability, low bitterness and the like. The product can be widely applied to food, sport food, curative healthcare food, protein drinks, diet food and novel fermented food.

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