The invention provides a galloyl glucose preparation method. The galloyl glucose preparation method includes steps of grinding, crude extraction, extraction, high-speed countercurrent chromatographic preconcentration, purification, collection, drying and the like. Compared with a traditional column chromatography method, the galloyl glucose preparation method has advantages of time saving, economy, quickness and high efficiency; according to actual requirements, chemical components of galloyl glucose can be obtained while 4-6 types of monomeric compounds of the galloyl glucose can be obtained by further purification. By the galloyl glucose preparation method, a new plant source for large-scale preparation of galloyl glucose compounds is provided, and experimental bases are provided for related drug development.