A demand side response-based micro-grid operation optimization method is disclosed and comprises the following steps: information of a micro-grid system to be optimized is collected; the information comprises all user power, power of a connecting line between a micro-grid and an external power grid, and renewable energy contribution; based on a principle of using new energy to a maximum extent, a load control aim of each controllable device owned by users is calculated via utilization of the above information; a load of each controllable device is controlled via a weighting coefficient queuing algorithm; if controllable devices owned by users of a green response grade, a yellow response grade and a red response grade are used while the load control aim is not attained, an energy storage device intervenes; the method also comprises other steps. The demand side response-based micro-grid operation optimization method provided in the invention is based on the weighting coefficient queuing algorithm and is suitable for controlling controllable loads such as air-conditioners, heat pumps and the like that are common in a home; the users are allowed to choose a corresponding response grade, and consideration is given to user comfort and fairness.