The present invention relates to the field of the electric power facilities, and specially discloses an insulation junction box for hot-line work. The insulation junction box comprises an insulation box with an opening at the upper end of the insulation box, and gaps arranged downwards from the upper ends are disposed at two opposite side surfaces of the insulation box; and insulation clamps are arranged at the gaps, a manual hydraulic clamp is arranged at any one of the two side surfaces, and the clamp heads of the manual hydraulic clamps are located in the insulation box. The insulation junction box for hot-line work is provided with an insulation box and insulation clamps, and a live line is fixed in the insulation box in the construction process without the risk of creeping down the live line, so that the safety of the hot-line work is greatly increased.