The present invention provides a pixel unit. The pixel unit includes a pixel definition layer, a pixel electrode, a light emitting layer and a common electrode. The pixel electrode comprises a bottom surface and a side surface. The side surface extends to a top of a side wall of the first through hole on the side wall of the first through hole. The light emitting layer is formed on the pixel electrode and covers over the pixel definition layer. The common electrode is formed on the light emitting layer and covers over the light emitting layer and extends on a top surface of the pixel definition layer. The present invention also provides a method of manufacturing a pixel unit and a display. The pixel unit according to the present invention greatly reduces scattering of a light from the light emitting layer to the surroundings to greatly alleviate color mixing phenomenon occurred between the pixel units so that the color mixing problem brought by scatting of the light is effectively solved, increases luminous area of the pixel unit to enhance overall luminance, cuts down the mask cost to significantly reduce the cost of the whole process, improves productivity and enhances the luminous effect.