The invention discloses a method for preparing graphene fibers with an air laying method. The method comprises the following steps of: spraying expanded graphite oxide worm powder into the surface of an air laid micro-pore nickel net through an airflow spraying method under the action of a negative-pressure air suction device; holding two sides of a mirror surface stainless steel film and the micro-pore nickel net, and pressing the expanded graphite oxide worm powder into a graphite oxide thin film under the action of a compression roller; cutting the graphite oxide thin film into continuous graphite oxide fibers; twisting the continuous graphite oxide fibers by spraying air through a fluid nozzle; and meanwhile, spraying a laser beam to a corresponding twisting point position through a hole formed in the tail end of the nozzle, and converting the graphite oxide fibers into the graphene fibers. The method is simple and convenient to operate, does not need subsequent treatment and impurity removal and is low in cost; the graphene fibers prepared by the method have the advantages of twisting degree, tight combination of lamellas, uniform surface and inner structures and excellent mechanical properties; a nano functional material can be coated before the graphite oxide thin film is cut and the functional composite graphene fibers are obtained.