The invention discloses a method for rapid detection of yellow fever virus by using isothermal nucleic acid amplification technology. The detection method comprises a recombinase mediation of an isothermal nucleic acid amplification system, a reaction buffer and detection of universal primers by yellow fever virus. The method is carried out under isothermal conditions (35-45 DEG C) within 10-20 minutes, so as to achieve amplification on yellow fever virus RNA. The detection method provided by the present invention has the advantages of high sensitivity, good specificity, simple and rapid operation and low quality requirements on the detection material, facilitates clinical applications, and is applicable to rapid detection of yellow fever virus.