Provided are a bacillus licheniformis high-temperature alpha-amylase mutant and application thereof.A nucleotide sequence of the high-temperature alpha-amylase mutant is shown as SEQ ID NO.1, and an amino acid sequence of the high-temperature alpha-amylase mutant is shown as SEQ ID NO.2.The mutant is obtained by conducting protein engineering improvement and selection on high-temperature alpha-amylase from bacillus licheniformis, the glucose content in a product of hydrolyzed starch can be improved and the dextrin and oligosaccharide content can be reduced by applying mutant enzyme in the hydrolyzed starch, the glucose content in syrup obtained through separate starch hydrolysis is 72%, the using amount of saccharifying enzyme in the follow-up further glucose production can be decreased, and reduction of the production costs is promoted.