The invention provides a queen bee storage box which comprises a bottom shell and a cover covering the bottom shell. A cavity is formed in the bottom shell, and a worker bee slot for allowing worker bees to get in and out is formed in the cover. The queen bee storage box is characterized in that a honey tank, a blocking sheet and an acting mechanism are further included, the honey tank is mounted in the cavity, a honey sucking hole is formed in the honey tank, the blocking sheet is controlled by the acting mechanism and is changed between an open position and a closing position relative to the honey sucking hole, a certain distance is kept between the blocking sheet and the honey sucking hole at the open position, and the honey sucking hole is covered with the blocking sheet at the closing position. The queen bee storage box is simple in structure and reasonable in design, queen bees can be conveniently managed in a centralized mode and can be conveniently dispatched, and feed is saved.