The invention provides a selection and distribution method of uplink piolet frequency in a massive MIMO system in a TDD mode. The method comprises the following steps: each cell interacting related piolet frequency sequence use information of a local cell to an adjacent cell, each cell base station interacting the uplink piolet frequency sequence use information and a DTX mode of a user in the local cell to the adjacent base station; the base station determining a user using one unlink piolet frequency sequence in the cell and a main sub-space of a channel matrix between the cell base stations, and the user using the same uplink piolet frequency sequence in the adjacent cell and the main sub-space of the channel matrix between the cell base stations; judging whether the user in the local cell can use the same uplink piolet frequency sequence with the user in the adjacent cell according to the relation of two main sub-spaces. The selection and distribution of the uplink piolet frequency reduces the uplink piolet frequency pollution of the massive MIMO system in the TDD mode through the information interaction between the user and the base station and the information interaction between the base stations.