The invention discloses an ultrasonic assistant electrostatic spinning device which comprises an ultrasonic assistant system, a supply system, a parameter control system, a collecting system and a glass box body, wherein the ultrasonic assistant system comprises an ultrasonic generator, an ultrasonic transducer, a hollow water tank and a low-temperature constant-temperature water bath tank; the ultrasonic transducer is fixed on the outer side of the hollow water tank and is controlled by the ultrasonic generator; the hollow water tank is connected with fixing screw nuts; two low-temperature constant-temperature water bath pipeline connectors are arranged at a side part of the hollow water tank; a water outlet is formed in the upper part of the hollow water tank; the supply system adopts a micro-injection pump to quantitatively push a boosting injector I; the head part of the boosting injector I is connected with the head part of a boosting injector II through a silicone tube; the handle part of the boosting injector II is connected with the handle part of a sample injector and is placed and fixed inside an outer thread straight tube, and the sample injector can be pushed at a same speed; the parameter control system is used for controlling the ambient temperature and the spinning voltage inside the glass box body; and the collecting disc of the collecting system is grounded and is driven by a motor to rotate. The ultrasonic assistant electrostatic spinning device is applied to long-term turbid liquid electrostatic pinning operation.