The invention discloses a processing method for a whole-bamboo flooring board. The commercial timber whole-bamboo flooring board is manufactured through the sequences of fresh Moso bamboo end truncating, bamboo splitting, removal of the inside-stick of bamboos, softening treatment, double-side thicknessing, drying, four-side planing, double-end milling, surface sanding, photo-curing wear-resistant paint coating, inspection and classification. According to the method, a high-temperature Moso bamboo softening and flattening technique is utilized, and the whole-bamboo flooring board is manufactured through treatment with special processes. Through the processing method, the utilization ratio of bamboos of the bamboo flooring board can be increased to more than 70%, and the cost of the raw material Moso bamboos of the whole-bamboo flooring board is greatly reduced. Therefore, the processing method has a wide market development prospect.