The invention discloses a dynamical staged safe regulating and controlling method for a mine ventilation system. According to a current obtained mine ventilation network wind amount optimizing and regulating scheme, an alternative combined safe regulating and controlling strategy of a main ventilator set and a wind window array is adopted, and besides, wind flow monitoring data in a regulating process are used for checking and correcting ventilation network branch wind resistance values, wind window wind resistance values and main fan characteristic parameters; an excavation replacement plan is combined for predicting the state change of a next stage mine ventilation system and a wind amount optimization regulating scheme, so that a complete regulating and controlling cycle is formed; under the premise that the ventilation system is not out of order, the situation that the wind amount of wind points where the wind amount is redundant is subjected to monotone decreasing to a desirable value is guaranteed; oppositely, the wind amount of the wind points where the wind amount is insufficient is subjected to monotone increasing to the desirable value. Therefore, the regulation of the mine ventilation system is safe and reliable. The dynamical staged safe regulating and controlling method disclosed by the invention can be used for not only on-site hand regulation, but also remote monitoring, so that the safety and the reliability of wind regulation of the mine ventilation system can be effectively improved.