The invention provides carrier protein His6-H4-NLS-Tat-AHNP and a preparation method.A wheat protein H4 is provided as DNA combination structural domain as the core area carrying treatment genes, and the core area is integrated with a nuclear localization signal area, a transmembrane structural domain Tat and Her2 target polypeptide, and resultantly the novel carrier protein used for gene treatment is produced.The carrier protein can effectively carry treatment genes into tumor cells, and express exogenous genes.The carrier protein His 6-H4-NLS-Tat-AHNP has no toxic effect on normal cells.Compared with conventional virus and chemical synthesis type carrier, the carrier protein is simple in preparation process, safe, non-toxic, easy to clear, and good in targeting and the like.