The invention relates to the technical field of the production of seamless transfer films, and in particular to a production method of a positioned gravure micro-character seamless transfer film. The method comprises the following steps: 1, according to the typesetting size requirements of printing, carrying out corresponding-dimension micro character photoetching on a holographic nickeltype and remaking an embossed hologram nickeltype; and 2, by adopting a double-plate-making roller mould pressing mode, heating a first plate-making roller by adopting two heating oil inlet/outlet pipelines and cooling by adopting eight cooling oil inlet/outlet pipelines; heating a second plate-making roller by adopting eight heating oil inlet/outlet pipelines and cooling by using two cooling oil inlet/outlet pipelines; in addition, carrying out fixed-point heating on cooling zones of the two cooling oil inlet/outlet pipelines while cooling the second plate-making roller by using the two cooling oil inlet/outlet pipelines at the same time. The positioned gravure micro-character seamless transfer film produced by adopting the method disclosed by the invention has the advantages of no ghosting phenomenon and micro character clearness and completeness, so that the anti-counterfeit function can be improved and the imitation difficulty is increased.