The invention provides a tympanum puncture and intratympanic injection medical device, and relates to the field of ear-nose-throat medical equipment, and mainly aims at solving the technical problems that in the prior art, medicine reflux is caused due to the fact that two holes are formed in secondary medicine injection through needle insertion after puncture and aspiration, and puncture pain of a patient is increased. According to the technical scheme, the tympanum puncture and intratympanic injection medical device comprises a first puncture device, a second puncture device, a suction device and an injection device; the first puncture device comprises a puncture needle and a first connector; the second puncture device comprises an injection needle and a second connector; the suction device comprises a negative pressure mechanism and a third connector; the injection device comprises an injector and a fourth connector; when the medical device is in a first using state, the third connector of the suction device is connected with the second connector of the second puncture device; when the medical device is in a second using state, the fourth connector of the injection device is connected with the second connector of the second puncture device.