The invention discloses reactive dye printing paste as well as a preparation method and an application thereof. The reactive dye printing paste is prepared from the following materials: sepiolite, kerosene, an emulsifying agent, urea, a reserve salt S, soda ash and water. The invention further provides an application of the reactive dye printing paste to printing and dyeing of towels and velvet fabrics. A preparation process of the reactive dye printing paste comprises the following steps: preparing the reactive dye printing paste, preparing color paste, printing a textile, drying, steaming, washing with water, soaping and drying; the reactive dye printing paste is formed by compounding the sepiolite and the emulsified kerosene; the sepiolite and the emulsified kerosene have good compatibility; and the formed color paste has good printing-through property and good stability of resistance to water and electrolyte and is low in cost, and the printing is ecological and environmentally friendly.