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发明授权
CN105694120B 一种壳寡糖透明质酸复合纳米微粒的制备方法 有权

一种壳寡糖透明质酸复合纳米微粒的制备方法
摘要：
本发明公开了一种高分子材料学领域的纳米微粒的制备技术，即一种壳寡糖透明质酸复合纳米微粒的制备方法，本发明采用壳寡糖和透明质酸作为原料，通过聚合物凝聚法合成目标产物。具体步骤为：将壳寡糖、透明质酸分别溶于去离子水中，得到壳寡糖溶液和透明质酸溶液，将上述两种溶液中的一种在搅拌条件下缓慢滴加到另一种溶液中，进行聚合物沉淀反应，待滴加量接近一半时，停止滴加，然后将所得的反应体系反向滴加到前一种溶液中，并保持搅拌。控制搅拌转速、壳寡糖的浓度、透明质酸的浓度、壳寡糖与透明质酸的质量比以及溶液的滴加速度，待反应完毕，超声分散，分离纯化，干燥得到本发明的产品。产品的平均粒径在120‑200nm，Zeta电位为‑20mV‑+30mV，可作为药物载体和医用生物材料，具有良好的研究和开发前景。
摘要（英）：

The invention discloses a preparation method of a chitosan oligosaccharide-hyaluronic acid composite nanometer particle and belongs to the field of polymers. The preparation method utilizes chitosan oligosaccharide and hyaluronic acid as raw materials and prepares a desired product through a polymer coacervation method. The preparation method comprises respectively dissolving chitosan oligosaccharide and hyaluronic acid in deionized water to obtain chitosan oligosaccharide and hyaluronic acid solutions, slowly and dropwisely adding one of the chitosan oligosaccharide and hyaluronic acid solutions into the other one so that the solutions undergo a polymer precipitation reaction, when a dropwise addition ratio is close to 50%, stopping dropwise addition, reversely and dropwisely adding the obtained reaction system into the added solution along with stirring under control of a stirring rotation rate, a chitosan oligosaccharide concentration, a hyaluronic acid concentration, a mass ratio of chitosan oligosaccharide to hyaluronic acid and a solution dropwise addition rate, after the reaction is finished, carrying out ultrasonic dispersion, carrying out separation purification and carrying out drying to obtain a product. The product has the average particle size of 120-200nm, Zeta potential of -20mV to +30mV, can be used as a drug carrier and a medical biomaterial and has a good research and development prospect.

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