The invention discloses a preparation method of acetyl protection 2,6-dideoxy alpha-glucose glycol glycosides. The method is characterized by comprising the steps that under the protection of nitrogen, an acylated 2,6-dideoxy glucose donor and an alcohol receptor are mixed according to the molar ratio of 1:(0.3-3), stirring is conducted, a molecular sieve and an organic solvent are added, a synthetic reaction of glycosylation is conducted under catalysis of boron trifluoride diethyl ether, after the reaction is finished, the molecular sieve is filtered out, filtrate is subjected to concentration and purification, and the obtained product is acetyl alpha-configuration glycol glycosides. Compared with the prior art, the preparation method of acetyl protection 2,6-dideoxy alpha-glucose glycol glycosides is simple in technology, convenient to operate, low in production cost, high in yield, good in stereoselectivity, mild in reaction condition, capable of avoiding use of high-toxic chemical raw materials, free of environmental pollution, green, environmentally friendly, economical and efficient and has an application prospect.