The invention discloses a detection primer set for reverse transcription loop-mediated isothermal amplification (RT-LAMP) of Noroviruses (NV), a detection method and a kit. The detection primer set comprises an upstream outer primer F3, a downstream outer primer B, an upstream inner primer FIP and a downstream inner primer BIP, all of which are shown as the SEQ ID NO. 1, 2, 3 and 4 respectively. The RT-LAMP technology for detecting NV is simple, specific, sensitive and suitable for field detection and simple laboratory detection, and a novel virus detection method is provided. During sample detection, the sensitivity of the RT-LAMP technology reaches 97.7% higher than 90.7% of RT-PCR, and the RT-LAMP technology is more suitable for rapid detection of field samples and rapid detection in a simple laboratory and can be widely applied to hospitals, the food industry, entry and exit inspection and quarantine, quality supervision and other sectors and fields.