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发明授权
CN105628937B 一种布鲁氏菌病A19-ΔVirB12分子标记疫苗免疫抗体与自然感染抗体鉴别方法 失效 - 权利终止

一种布鲁氏菌病A19-ΔVirB12分子标记疫苗免疫抗体与自然感染抗体鉴别方法
摘要：
本发明公开了一种布鲁氏菌病A19‑ΔVirB12分子标记疫苗免疫抗体与自然感染抗体鉴别方法，包括：a、根据布鲁氏菌A19的VirB12基因特性，以生物信息学软件分析获得活性序列，对应的DNA序列为：多肽074DNA序列：GGCCTGACGGACAACAACTGCCCTCCTCCCGGTGATACGCAA；b、以DNA序列为模板，将步骤a中的序列通过人工合成，得到多肽，其氨基酸序列为；多肽074：GLTDNNCPPPGDTQ；c、以步骤b中的活性多肽成分作为包被抗原即可。本发明解决了免疫动物与临床患病动物难以鉴别的问题，对牛布鲁氏菌病的防控、根除和净化具有实际应用价值。
摘要（英）：

The invention discloses an immunology identification method for Brucella A19-delta VirB12 molecular marker vaccine. The method comprises the following steps: a, carrying out bioinformatics software analysis according to the VirB12 gene characteristics of Brucella A19, so as to obtain an active sequence, wherein the corresponding DNA sequence is a polypeptide 074 DNA sequence: GGCCTGACGGACAACAACTGCCCTCCTCCCGGTGATACGTACGCAA; b, conducting artificial synthesis on the sequence obtained in the step a by taking the DNA sequence as a template, so as to obtain polypeptide, wherein the amino acid sequence of the polypeptide is a polypeptide 074 amino acid sequence: GLTDNNCPPPGDTQ; c, taking the active polypeptide ingredient obtained in the step b as an envelope antigen. According to the method, the problem that an immune animal and a clinic sick animal are difficult to identify is solved, and the practical application value in preventing, controlling, eliminating and purifying cattle brucellosis is high.

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