The invention discloses an anti-seepage water-stop structure for a hydraulic engineering dam. The anti-seepage water-stop structure comprises a dam body. The bottom face of the dam body is connected with a dam foundation anti-seepage layer through an anti-seepage connection layer. The upper bottom face and the lower bottom face of the anti-seepage connection layer are both step-shaped contact faces. The upper bottom face is poured in the dam body. The lower bottom face is poured in the dam foundation anti-seepage layer. The dam body is provided with a dam body anti-seepage structure which is smeared with an antifouling paint layer and comprises a dam body anti-seepage layer. A bamboo fiber woven layer is connected outside the dam body anti-seepage layer in a sleeving mode. A concrete reinforcement layer is connected outside the bamboo fiber woven layer in a sleeving mode. The bamboo fiber woven layer is formed by intertwining bamboo fiber strips and lining gridding strips with the adoption of the combination mode of circular and longitudinal plane intertwining through a resin adhesive solution evenly and continuously. According to the anti-seepage water-stop structure for the hydraulic engineering dam, the step-shaped dam body anti-seepage layer extending from the dam top to the dam bottom is arranged on the side of a water-blocking face of the dam body so that the permeation, scour, erosion and abrasion effects of a water body on the surface of the dam body can be effectively prevented, and thus the anti-seepage property and durability of the whole dam and a foundation are ensured.