The invention provides a preparation for preventing the parthenocissus tricuspidata leaf spot disease and belongs to the field of garden biological preparations.The preparation is prepared from pinnately compound leaves of radix astragali, petals of platycodon grandiflorum, perilla leaves, glutinous rice roots, petals of amomum cardamomum, dried orange peel, dried hawthorn fruit, aconitum carmichaeli leaves, dried oyster meat, pomegranate peel, pepper branches, loofa sponge, cactus flesh and spinach roots.The raw materials are placed in water to be decocted to prepare the preparation.Experiments show that the preparation has a good prevention effect on the parthenocissus tricuspidata leaf spot disease, is little in environment pollution, simple in manufacturing method and suitable for being widely used.