The invention provides a dynamic non-contact cell mechanical stimulation loading device. A plurality of pipelines are formed in a wall of a culture cavity to be communicated with a circulation water bath; the culture cavity is fixed in a box body, and the box body is filled with heat insulation materials; the output of an air compressor and a CO2 pot is input into the culture cavity after being subjected to matching control through a gas flowmeter; a plurality of humidifying vessels are placed in the culture cavity, and a humidity measuring instrument is used for detecting the humidity inside the culture cavity; two same rotary plates rotate around a perpendicular rotating shaft under the drive of a motor, each rotary plate is provided with a permanent magnet, an object stage is installed between the two rotary plates, an experiment object is placed on the object stage, and the object stage is fixed instead rotating. Various dynamic non-contact cell mechanical stimulation loading ways can be achieved, space non-contact extrusion type, periodic gradient type and multi-dimensional uniform type mechanical stimulation effects are applied to cells, and an in-vitro cell culture environment is built for the cells.