The invention provides a naked-eye 3D display method, a display system and a simulation analysis method of the display system. The display system comprises an image display layer, a lens array film layer, a backlight source and a human eye detection module. The lens array module is arranged between the image display layer and the backlight source; the image display layer comprises at least one display unit; the backlight source comprises at least one light-emitting module; the at least one display unit is in one-to-one correspondence with the at least one light-emitting module; for the system comprising the multiple display units, shading layers are arranged between the light-emitting modules to reduce crosstalk rate; the human eye detection module is connected with the light-emitting modules; and the human eye detection module is used for detecting the observation state of the eye of an observer, and selecting the light-emitting module corresponding to an appropriate visual area for projection according to the observation state of the observer. The naked-eye 3D display system can select appropriate light-emitting unit combination by detecting the spatial position and observation angle of the eye of the observer with respect to the image display layer, so that the human eye can obtain an optimal watch effect.