The invention provides a suppressor cell system of the porcine pseudorabies virus. The system can be used for preparing a model of a cell or an animal not infected by the pseudorabies virus. A corresponding preparation method is provided. The prepared suppressor cell system of the porcine pseudorabies virus comprises a Cas9 endonuclease system and sgRNA of a UL30 gene of a target PRV. The cell system has a remarkable inhibiting function on PRV infection, can be used for preparing the model of the cell or the animal not infected by the pseudorabies virus, and has wide application prospects in clinical and laboratory research.