The invention discloses a method for promoting ecdysis of a giant salamander and a method for extracting a giant salamander genome. The method pushes the giant salamander to generate exuviae quickly through environmental stimulus, and by means of the method, giant salamander exuviae can be collected quickly and effectively, materials are provided for extracting the giant salamander genome, a path of extracting the giant salamander genome for genetics analysis is added, the giant salamander is effectively prevented from being hurt, the purpose of protecting the giant salamander is achieved, and the method also conforms to the animal protection concept. The method is simple and easy to implement, low in cost and good in using effect.