The invention discloses a process method for immobilizing lipase B from candida antarctica fermented supernatant fluid. According to the method, the immobilized candida antarctica lipase B is prepared by using a cheaper vector, adopting a water-phase immobilization mode and cooperating with a post-treatment process, therefore, the enzyme activity recovery rate under water-phase adsorption is increased, the cost and the using effect are balanced, and the important significance on industrial application of the CALB is achieved.