The present invention discloses a preparation method of a flexible film with super hydrophobicity and high adhesion. The preparation method comprises the steps of: adding polyvinylpyrrolidone, tetrabutyl titanate and acetic acid into ethanol and stirring to obtain a mixed solution; subjecting the mixed solution to electrostatic spinning to obtain a nanofiber film; drying and carbonizing the nanofiber film to obtain a TiO2 / C film; subjecting the TiO2 / C film to a hydrothermal treatment in a sodium hydroxide solution, and conducting H<+> exchange, drying and heat treatment to obtain a TiO2(B)/C film; treating the TiO2(B)/C film in a toluene solution containing perfluorooctyl trichlorosilane to obtain a flexible film with super hydrophobicity and high adhesion. The invention also discloses a flexible film with super hydrophobicity and high adhesion. The flexible film has excellent super hydrophobicity, high adhesion performance, and good flexibility, and has great application potential in the preparation of biomedical and microfluidic devices.