The invention provides a preparation method of a metal egg tray interlayer structure. The metal egg tray interlayer structure is plate-shaped or tubular, and the preparation method of the metal egg tray interlayer structure comprises the following steps: step 1, setting connecting points (2) for connection and fixation on the surface of metal foil (1) which is taken as the core material, wherein on the surface of the metal foil (1), the area without the connecting points (2) is a non-connection area (3); coating the non-connection area (3) with an antiwelding agent or antiwelding-foaming agent; the antiwelding-foaming agent is formed by adding foaming agents like titanium hydride into the antiwelding agent. The preparation method provided by the invention proposes that the metal egg tray interlayer structure can be prepared by adopting the diffusion welding and superplastic forming technology, therefore on one hand, the appearance of parts after forming, especially some titanium alloy and aluminum alloy egg tray structures with complex appearance, can be precisely controlled, on the other hand, the technical problem that the titanium alloy and aluminum alloy egg tray interlayer is difficult in forming is solved, and the relatively high bonding strength among diffusion bonding interfaces is further achieved.