Provided is a preparation method of an amide keyed ferrocenyl nano-porous polymer. The preparation method comprises the steps that 5% to 15% of first construction unit, 10% to 20% of second construction unit and 75% to 90% of dispersion medium are added to a container in sequence, ultrasonic dispersion is carried out for 30 min under inert gas shielding, the container is filled with inert gas, the temperature is slowly raised to 150 DEG C within 2 h, and constant-temperature reacting is carried out for 72 h; after reacting is finished, filtering is carried out, and washing is carried out four times through chloroform, distilled water, methyl alcohol and acetone; Soxhlet extraction is carried out for 48 hr through methyl alcohol, and then a product is subjected to vacuum drying for 24 hr at the temperature of 70 DEG C. The ferrocenyl nano-porous high polymer material is prepared through the one-step method, the material has the large specific surface area and pore volume and good chemical stability and thermal stability, and the material has potential application on the aspects of adsorption, gas storage, sewage treatment, catalysis, gas separation and fluorescence sensing.