The present invention discloses a method for improving effective ingredients of breviscpini by applying a molybdenum fertilizer, and belongs to the technical field of traditional Chinese medicine planting. The main technical scheme is as follows: taking 2-month-old breviscpini seedlingsto perform transplantation; after planting the seedlings in 20-25 days, 30-35 days and 40-45 days, applying a molybdenum fertilizer diluted by 750-800 times in each period, wherein the molybdenum fertilizer applying amounts are respectively 160 g/mu, 200 g/mu and 240 g/mu; and when the flowering rate of the breviscpini reaches 40%, collecting the above-ground part of the plants to perform fast drying. The method is simple in operation and low in fertilizing amount, so that the contents of effective ingredients of breviscpini, scutellarin and caffeic acid ester are effectively improved.