The invention discloses a tissue culture method for cymbidium tortisepalum and application thereof. The method comprises the steps of taking cymbidium tortisepalum aseptic seedling stem tip as an explant, taking a proliferation medium to induce rhizomes to proliferate, taking an LED (light-emitting diode) as a tissue culture light source, and culturing by using blue light; then transferring the obtained rhizomes to a subculture growth medium, inducing multiple shoots to grow and a seedling to quickly develop, taking the LED as the tissue culture light source, and culturing by using red light; inoculating the newly generated seedling to a rooting medium for strong seedling rooting culture, taking the LED as the tissue culture light source, and culturing by using mixed red light and blue light which are matched according to a ratio of (2 to 4):(6 to 8). By adopting the tissue culture method, the problems of low sprouting ratio, slow growth, difficult rooting and the like existing in the prior tissue culture technology are effectively solved; by using LED lamps as the tissue culture light source, 30 to 50 percent of electricity resource is saved, the cost is saved, and the efficiency of industrialized production is improved.