The invention discloses a cultivation method and applications of vegetables and fruits capable of expressing human amylin. Human amylin gene is digested by KpnI and XbaI and then is loaded on a plant expression vector CAMV-1301 to construct a plant expression vector pcambia-CAMV35S-hIAPP-NOS-1301 of anti-kanamycin gene. Through a pollen tube path way method, amylin gene is successfully converted into the F1 generation of selfing line of vegetables and fruits, and transformed plants that express amylin gene are obtained at the same time. The provided cultivation method can successfully convert human amylin gene into vegetables and fruits. By eating the vegetables and fruits, which contains an important protein hormone namely amylin for regulating the glycometabolism of human, the diabetes can be prevented and the diabetes treatment can be assisted through diet, and thus the problems of drug resistance and adverse reactions caused by drugs are solved. Moreover, the economic burden, mental pressure, and various adverse reactions caused by medicine injection and oral taking of medicines are relieved for the diabetes patients.