The invention provides a disposable automatic cut-off negative pressure conduction module of gravity venous transfusion and especially relates to an automatic cut-off negative pressure conduction module of gravity venous transfusion, wherein a liquid inlet pipe is larger than a liquid outlet pipe, the module can be connected and assembled between a bottle-through needle and a drip chamber by an upper transfusion pipe, the module can be automatically cut off by a diaphragm when medicine transfusion is completed, and negative pressure information can also be transmitted by the module. The automatic cut-off negative pressure conduction module of the gravity venous transfusion provided by the invention is advantageous in that a negative pressure value is maximum, no safety hidden danger exists, utilization is convenient and manufacture is simple. The automatic cut-off negative pressure conduction module of the gravity venous transfusion can be widely applied in multiple kinds of disposable transfusion and blood transfusion appliances such as disposable venous transfusion devices and special transfusion devices.