The invention belongs to the technical field of preparation of display panels, and relates to a preparation method of a 3D folding information display panel. The method comprises the following steps: firstly, designing a display panel text plan, carrying out information editing and typesetting of display panel content by Photoshop CS5 software in text inputting, pattern drawing, material clipping and special effect editing manners, and then carrying out pattern and information printing; and finally carrying out folding and fabricating on the printed display panel. The prepared display panel adopts two forms, namely an overall single-sided panel and a center line folding panel; the content of each display panel can be divided into image-text information and a 3D foldable part; the preparation method is simple in preparation technology, convenient to operate, small in occupied space of the display panel, low in exhibition cost, good in display effect, short in exhibition arrangement time and wide in application.