The invention belongs to the technical field of vaccine preparation, and in particular discloses a trichomonas vaginalis alpha-actinin recombinant fusion protein immunoprotecive antigen, a vaccine and application. Aprokaryotic expression system is used to construct an expression strain capable of stable expression of alpha-actinin subunit fragment in a large amount, and the expression strain is purified to obtain an ACT-T protein; the protein as an antigen infects mice with trichomonas vaginalis, and the mice recover after 14 days; the antigen has immune protective effect on mice close to 100%. The ACT-T fusion protein as antigen is prepared into a subunit vaccine, so as to realize the protective immunity in mice. The vaccine has widespread application value.