The invention relates to the biological technology field, and provides a preparation method for an ELISA detection kit of goat corynebacterium pseudotuberculosis antibodies. Complete corynebacterium pseudotuberculosis thalluses are employed as coating antigens, an indirect ELISA detection method is established, whether serum contains corynebacterium pseudotuberculosis antibodies is detected and whether a goat is infected with caseous lymphadenitis is determined. The positive and negative critical value is determined, and the detection kit has high specificity and repetition, can detect pseudotuberculosis antibodies in goat serum rapidly and effectively, and can be used for goat pseudotuberculosis diagnosis and epidemiological investigation.