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发明授权
CN105181423B 碱性磷酸酶(NAP)染色液(化学染色法) 有权

碱性磷酸酶(NAP)染色液(化学染色法)
摘要：
本发明涉及生物技术领域，公开了一种中性粒细胞碱性磷酸酶染色试剂盒以及染色方法。本发明所述试剂盒包括pH值为9.2‑9.8的碱性磷酸酶底物溶液，所述碱性磷酸酶底物为N‑(2‑甲基‑5‑甲氧基‑4‑联苯基)‑3‑磷酸氧基‑2‑萘甲酰胺。本发明以碱性磷酸酶底物替代现有萘酚AS‑BI磷酸钠盐、萘酚AS‑MX磷酸钠盐等进行NAP染色，该底物化学结构中通过引入发色基团联苯基，增强碱性磷酸酶水解底物产生的萘酚与偶氮盐反应的显色强度，从而大大提高了碱性磷酸酶的显色灵敏度和阳性染色。
摘要（英）：

The invention relates to the technical field of biology, and discloses a neutrophile granulocyte alkaline phosphatase staining kit and a staining method. The kit comprises an alkaline phosphatase substrate solution with the pH value being 9.2-9.8. The alkaline phosphatase substrate is N-(2-methyl-5-methoxyl-4-xenyl)-3-phosphoric acid oxygroup-2-naphthamide. The alkaline phosphatase substrate replaces existing naphthol AS-BI sodium phosphates, naphthol AS-MX sodium phosphates and the like for conducting NAP staining, chromophoric group xenyl is introduced in the chemical structure of the substrate, the reacting color intensity of naphthol produced by hydrolysis of the substrate by the alkaline phosphatase and tetrazonium salt is enhanced, and therefore the color display sensitivity and positive staining of the alkaline phosphatase are greatly improved.

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