The invention discloses a decoloring method of natural colored animal fibers. The method comprises the following steps: 1, preprocessing; 2, carrying out first decoloring; 3, rinsing; 4, carrying out second decoloring; 5, carrying out third decoloring; and 6, carrying out air drying treatment. The method overcomes disadvantages of the prior art, and reduces damages of the decoloring process to the animal fibers.