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发明授权
CN105063034B 分子定量分析草珊瑚与3种混淆品的特异性引物及方法 有权

分子定量分析草珊瑚与3种混淆品的特异性引物及方法
摘要：
本发明提供一种分子定量分析草珊瑚与3种混淆品的特异性引物及方法，属于生物技术领域，特异识别草珊瑚的上游特异性引物SgF：5’‑ TGATTCTGATAGATT‑TTTGAAGAATTGA ‑ 3’与下游引物trnL‑Fb；同时特异性识别金粟兰、及己和宽叶金粟兰的特异上游引物ChF：5’‑ ATCTGATCACCCTTACACTTACAAGC ‑ 3’与下游引物trnL‑Ff。通过这2对引物构建构建荧光定量PCR，进行混合样品的中特定样品的分子定量分析。本发明提供一种可准确，快速，简单定量分析草珊瑚与金粟兰、宽叶金粟兰和及己3种混淆品的技术，为相关草珊瑚饮片与这3种混淆品的混合品定量分析提供技术支持。
摘要（英）：

The invention provides a specific primer and method for molecular quantitative analysis of sarcandra glabra and three kinds of adulterant and belongs to the field of biotechnology. An upstream specific primer SgF:5'-TGATTCTGATAGATT-TTTGAAGAATTGA-3' and a downstream primer trnL-Fb of sarcandra glabra are specifically recognized; a specific upstream primer ChF:5'-ATCTGATCACCCTTACACTTACAAGC-3 and a downstream primer trnL-Ff of chloranthus spicatus, radix chloranthi serrati and henryi hemsl are specifically recognized. A fluorogenic quantitative PCR is built through the two pairs of primers, and molecular quantitative analysis of a specific sample in mixed samples is carried out. The technology capable of analyzing sarcandra glabra and adulterant of chloranthus spicatus, henryi hemsl and radix chloranthi serrate accurately, fast, easily and quantitatively is provided, and technical supports are provided for quantitative analysis of the adulterant of related sarcandra glabra decoction pieces, chloranthus spicatus, henryi hemsl and radix chloranthi serrate.

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