The invention discloses a kit used for detecting influenza viruses and an application thereof. The kit comprises a primer pair set used for detecting the influenza viruses. The primer pair set is composed of four primer pairs, and the sequences of the primer pair set are the first sequence to the eighth sequence in the sequence table. According to the kit, the high throughput is supported, and influenza virus infections can be rapidly and accurately detected; in the clinical aspect, the detection results of four influenza virus indexes can be obtained in one hour, and the kit is rapider than a real-time fluorescence quantification PCR method commonly adopted at present, and is of great significance in rapid treating and pharmacy guiding assisting. Meanwhile, multi-index detection can be used for regional epidemiology investigating and surveying and regional epidemic surveillance to research the epidemic condition of the influenza virus infections in China.