The invention relates to a manufacturing technology for antibiosis wood fiber socks. After opening and purifying are conducted on wood fibers through an opener, cone yarn is manufactured; the cone yarn is woven into gray fabric preparing for manufacturing the socks; the gray fabric is soaked into a hot water groove, and water-soluble filaments are flushed and removed; the gray fabric is boiled and bleached; the boiled and bleached gray fabric is dyed; the dyed gray fabric is dried and ironed through a drying device; the gray fabric is put into an antibiosis solution to be soaked; the soaked gray fabric is cut and sewn, and the wood fiber socks are obtained. According to the manufacturing technology for the antibiosis wood fiber socks, after the antibiosis processing, breeding of bacteria can be effectively prevented, the socks cannot be stained with microorganisms, the characteristics of the water absorption property, the soft hand touch and the like are maintained, and the socks are good in moisture absorption and sweat releasing performance, excellent in high-temperature resisting performance and stink absorbing and sewage discharging performance, environmentally friendly, long in service life, simple in processing technology and high in production efficiency.