The invention discloses a phenylthiocarbamide solution concentration detecting device and method. The device comprises a computer, a collecting unit and a collecting workbench. The collecting unit comprises a cell electrode plate, an acoustic surface wave resonator and a conversion unit. The collecting workbench comprises a base, the base is provided with a containing groove and a lifting device, a liquid dropping chamber is formed above the containing groove and connected with the lifting device through a connecting mechanism, a micro pump communicated with the liquid dropping chamber is arranged at the bottom of the liquid dropping chamber, a liquid dropping head communicated with the micro pump is arranged at the bottom of the micro pump, a liquid inlet is formed in the top of the liquid dropping chamber, a washing mechanism and a draining mechanism are arranged in the liquid dropping chamber, a disk is arranged above the liquid dropping chamber, a plurality of liquid storage cavities are formed around the circle center in the disk, a liquid outlet corresponding to the liquid inlet in position is formed in the bottom of the corresponding liquid storage cavity, a liquid outlet electromagnetic valve is arranged at the liquid outlet, and a driving motor driving the disk to rotate is arranged on the top of the liquid dropping chamber. According to the phenylthiocarbamide solution concentration detecting device and method, the structure is simple, cost is low, and the phenylthiocarbamide solution concentration can be quickly and accurately detected.