知搜-全球专利检索

  1. 登录
  2. 注册
发明公开
CN104950110A 激酶活性测定方法、ATP衍生物及其用途及制造方法 失效 - 放弃专利权

激酶活性测定方法、ATP衍生物及其用途及制造方法
摘要：
本发明提供激酶活性的测定方法，其包括：使激酶、底物和含DNP基的ATP衍生物反应而向上述底物导入DNP基的工序，混合含有DNP基的底物和抗DNP抗体的工序，及检测形成的复合物而测定激酶的活性的工序，其中上述ATP衍生物是二硝基苯基经接头与ATP的γ位的磷酸基结合的化合物。
摘要（英）：

Disclosed is a method for determining kinase activity. The method comprises: (A) mixing a specimen that comprises a kinase, a substrate of the kinase and an adenosine triphosphate (ATP) derivative that comprises a dinitrophenyl (DNP) group to obtain a mixture that comprises a DNP group-containing substrate in which the DNP group is introduced in the substrate; (B) mixing the mixture obtained at the (A) and an antibody that binds to the DNP group to form a complex that comprises the DNP group-containing substrate and the antibody; and (C) determining an activity of the kinase by detecting the complex. The ATP derivative is a compound in which the DNP group is bound to a phosphate group at a gamma position of ATP via a linker.

0/0