The invention provides a body fluid collection and storage device and a use method thereof. The device comprises a storage body and a blocking object and further comprises a sealing device. The storage body is used for storing storage liquid and body fluid. The blocking object is used for blocking the storage body. The sealing device comprises a sealing pipe and an annular sealing piece which are arranged in the storage body. The storage body is partitioned into a first sealing cavity and a second sealing cavity through the annular sealing piece. The sealing pipe is movably inserted into the annular sealing piece to enable the first sealing cavity and the second sealing cavity to be communicated or isolated in a sealed mode. According to the method for collecting and storing the body fluid through the device, the number of the operation steps can be reduced, the user experience is improved, the quality of the collected body fluid can be guaranteed accordingly, and inspection accuracy is improved.