The invention discloses a microfluidic ice valve with high response speed. The valve comprises a microfluidic chip, an anti-freezing solution injector and a refrigeration unit, wherein the anti-freezing solution injector is arranged below the microfluidic chip, and is fixed on the refrigeration unit; heat conducting silicone grease is coated on the contacted surfaces of the anti-freezing solution injector and the refrigeration unit; and the refrigeration unit and the anti-freezing solution injector can move corresponding to the microfluidic chip. The microfluidic ice valve with high response speed has the following beneficial effects: (1) the response time is shortened; (2) the integration density is improved; (3) the limit flowing speed rises; (4) the performance of the valve is not limited by a chip material; and (5) the cost is reduced.