The invention discloses a cardiac muscle tissue engineering product and its preparation method and use. The preparation method comprises 1, omentum majus decellularized matrix scaffold material preparation: immersing omentum majus in a decellularizing liquid containing lauryl sodium sulfate, DNA enzyme and trypsin so that decellularizing treatment is realized and 2, myocardial cell-omentum majus decellularized matrix complex construction and culture. The cardiac muscle tissue engineering product comprises the myocardial cell and omentum majus decellularized matrix scaffold material. The omentum majus decellularized matrix has a good 3D netted space structure and is rich in a growth factor so that myocardial cell growth and nutrient and metabolite transport are realized. The cardiac muscle tissue engineering product has an important meaning for cardiac muscle damage restoration based on the cardiac muscle tissue engineering product and cardiac muscle treatment drug screening utilizing the cardiac muscle tissue engineering product as a model.