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发明公开
CN104862267A 适应于无血清培养和悬浮培养的MDCK来源的细胞系与利用该细胞制备疫苗病毒的方法 有权 转让

适应于无血清培养和悬浮培养的MDCK来源的细胞系与利用该细胞制备疫苗病毒的方法
摘要：
本发明公开了一种马丁达比犬肾传代细胞(MDCK)来源的细胞系。该MDCK来源的细胞系来源于保藏的编号为ATCC CCL-34的MDCK细胞。所述MDCK来源的细胞系可以通过无血清培养和悬浮培养制备。优选地，所述MDCK来源的细胞系具有低致瘤性或无致瘤性。优选所述MDCK来源的细胞系选自MDCK Sky1023、MDCK Sky10234和MDCK Sky3851。进一步公开的是培养所述MDCK来源的细胞的培养方法以及利用所述MDCK来源的细胞制备疫苗病毒的方法。
摘要（英）：

Disclosed is a Madin-Darby canine kidney (MDCK)-derived cell line. The MDCK-derived cell line is derived from MDCK cells deposited under accession number ATCC CCL-34. The MDCK-derived cell line can be prepared by serum-free culture and suspension culture. Preferably, the MDCK-derived cell line has low or no tumorigenicity. The MDCK-derived cell line is preferably selected from MDCK Sky1023, MDCK Sky10234 and MDCK Sky3851. Further disclosed are a culture method for growing the MDCK-derived cells and a method for producing a vaccine virus using the MDCK-derived cells.

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