The invention discloses a method for extracting taxol from a Hainan Pestalotiopsis sp. F metabolite. The method comprises the steps of thallus culturing and fermentation; secondary metabolite extraction; and column chromatography purification. Taxol produced by the extracted Hainan Pestalotiopsis sp. F has a same structure with standard taxol, and the output is 1466.87[mu]g/L. The taxol output of the above fungus in panda scurf is substantially higher than that of the fungus from other animals. Production of taxol from the fungus may be a potential candidate method for industrial large-scale production of taxol.